{"id":4824,"date":"2025-03-21T15:47:30","date_gmt":"2025-03-21T07:47:30","guid":{"rendered":"https:\/\/www.zuheliao.com\/index.php\/archives\/4824"},"modified":"2025-03-21T15:47:30","modified_gmt":"2025-03-21T07:47:30","slug":"%e5%9b%9b%e7%94%b2%e5%9f%ba%e4%b9%99%e4%ba%8c%e8%83%batemed%e5%9c%a8%e7%94%9f%e7%89%a9%e5%8c%96%e5%ad%a6%e5%ae%9e%e9%aa%8c%e4%b8%ad%e7%9a%84%e5%82%ac%e5%8c%96%e4%bd%9c%e7%94%a8%e5%8f%8a%e5%85%b6","status":"publish","type":"post","link":"https:\/\/www.zuheliao.com\/index.php\/archives\/4824","title":{"rendered":"\u56db\u7532\u57fa\u4e59\u4e8c\u80fa(TEMED)\u5728\u751f\u7269\u5316\u5b66\u5b9e\u9a8c\u4e2d\u7684\u50ac\u5316\u4f5c\u7528\u53ca\u5176\u5e94\u7528"},"content":{"rendered":"

\u56db\u7532\u57fa\u4e59\u4e8c\u80fa\uff08TEMED\uff09\uff1a\u751f\u7269\u5316\u5b66\u5b9e\u9a8c\u4e2d\u7684\u201c\u5e55\u540e\u63a8\u624b\u201d<\/h1>\n
\u5728\u751f\u7269\u5316\u5b66\u5b9e\u9a8c\u5ba4\u4e2d\uff0c\u6709\u4e00\u79cd\u770b\u4f3c\u4e0d\u8d77\u773c\u5374\u4e0d\u53ef\u6216\u7f3a\u7684\u5c0f\u5206\u5b50\uff0c\u5b83\u5982\u540c\u4e00\u4f4d\u9ed8\u9ed8\u65e0\u95fb\u7684\u201c\u50ac\u5316\u5242\u5927\u5e08\u201d\uff0c\u603b\u662f\u5728\u5173\u952e\u65f6\u523b\u53d1\u6325\u4f5c\u7528\uff0c\u5374\u53c8\u4f4e\u8c03\u5f97\u8ba9\u4eba\u5bb9\u6613\u5ffd\u7565\u5b83\u7684\u5b58\u5728\u3002\u5b83\u5c31\u662f\u56db\u7532\u57fa\u4e59\u4e8c\u80fa\uff08N,N,N’,N’-Tetramethylethylenediamine\uff09\uff0c\u7b80\u79f0TEMED\u3002\u5982\u679c\u4f60\u66fe\u7ecf\u53c2\u4e0e\u8fc7\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff08PAGE\uff09\u5b9e\u9a8c\uff0c\u90a3\u4e48\u4f60\u4e00\u5b9a\u548c\u8fd9\u4f4d\u201c\u5e55\u540e\u82f1\u96c4\u201d\u6253\u8fc7\u4ea4\u9053\u3002\u672c\u6587\u5c06\u5e26\u4f60\u6df1\u5165\u4e86\u89e3TEMED\u5728\u751f\u7269\u5316\u5b66\u5b9e\u9a8c\u4e2d\u7684\u50ac\u5316\u4f5c\u7528\u53ca\u5176\u5e7f\u6cdb\u7684\u5e94\u7528\u573a\u666f\uff0c\u5e76\u901a\u8fc7\u901a\u4fd7\u6613\u61c2\u7684\u8bed\u8a00\u548c\u751f\u52a8\u7684\u6bd4\u55bb\u4e3a\u4f60\u63ed\u5f00\u5b83\u7684\u795e\u79d8\u9762\u7eb1\u3002<\/p>\n

TEMED\u7684\u57fa\u672c\u7279\u6027\u4e0e\u7ed3\u6784<\/h2>\n
\u9996\u5148\uff0c\u8ba9\u6211\u4eec\u4ece\u79d1\u5b66\u7684\u89d2\u5ea6\u8ba4\u8bc6\u4e00\u4e0bTEMED\u3002\u5b83\u7684\u5316\u5b66\u5f0f\u4e3aC6H16N2\uff0c\u5206\u5b50\u91cf\u4e3a116.20 g\/mol\u3002\u8fd9\u79cd\u5316\u5408\u7269\u662f\u7531\u4e24\u4e2a\u7532\u57fa\u53d6\u4ee3\u7684\u6c28\u57fa\u901a\u8fc7\u4e00\u4e2a\u4e9a\u4e59\u57fa\u6865\u8fde\u63a5\u800c\u6210\uff0c\u5177\u6709\u5178\u578b\u7684\u4e8c\u80fa\u7ed3\u6784\u3002\u6b63\u56e0\u5982\u6b64\uff0cTEMED\u4e0d\u4ec5\u662f\u4e00\u79cd\u6709\u673a\u78b1\uff0c\u8fd8\u5177\u5907\u826f\u597d\u7684\u4eb2\u6838\u6027\u548c\u5f3a\u8fd8\u539f\u6027\uff0c\u8fd9\u4f7f\u5f97\u5b83\u5728\u8bb8\u591a\u751f\u5316\u53cd\u5e94\u4e2d\u6210\u4e3a\u7406\u60f3\u7684\u50ac\u5316\u5242\u3002<\/p>\n

\u4ea7\u54c1\u53c2\u6570\u4e00\u89c8\u8868<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u53c2\u6570\u540d\u79f0<\/th>\n \u6570\u503c\u6216\u63cf\u8ff0<\/th>\n<\/tr>\n<\/thead>\n
\u5316\u5b66\u5f0f<\/td>\n C6H16N2<\/td>\n<\/tr>\n
\u5206\u5b50\u91cf<\/td>\n 116.20 g\/mol<\/td>\n<\/tr>\n
\u5916\u89c2<\/td>\n \u65e0\u8272\u81f3\u6de1\u9ec4\u8272\u6db2\u4f53<\/td>\n<\/tr>\n
\u6c14\u5473<\/td>\n \u7c7b\u4f3c\u6c28\u7684\u523a\u6fc0\u6027\u6c14\u5473<\/td>\n<\/tr>\n
\u5bc6\u5ea6<\/td>\n \u7ea60.83 g\/cm\u00b3<\/td>\n<\/tr>\n
\u7194\u70b9<\/td>\n -45\u00b0C<\/td>\n<\/tr>\n
\u6cb8\u70b9<\/td>\n 175\u00b0C<\/td>\n<\/tr>\n
\u6eb6\u89e3\u6027<\/td>\n \u6613\u6eb6\u4e8e\u6c34\u3001\u9187\u7b49\u6781\u6027\u6eb6\u5242<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\u4ece\u4e0a\u8868\u53ef\u4ee5\u770b\u51fa\uff0cTEMED\u662f\u4e00\u79cd\u4f4e\u7c98\u5ea6\u7684\u6db2\u4f53\uff0c\u6613\u4e8e\u64cd\u4f5c\u4e14\u6eb6\u89e3\u6027\u826f\u597d\uff0c\u8fd9\u4e9b\u7279\u6027\u4f7f\u5176\u975e\u5e38\u9002\u5408\u7528\u4e8e\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e0b\u7684\u5404\u79cd\u6eb6\u6db2\u914d\u5236\u3002<\/p>\n
\u5728PAGE\u5b9e\u9a8c\u4e2d\u7684\u50ac\u5316\u4f5c\u7528<\/h2>\n
\u5728\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff08PAGE\uff09\u5b9e\u9a8c\u4e2d\uff0cTEMED\u626e\u6f14\u7740\u81f3\u5173\u91cd\u8981\u7684\u89d2\u8272\u3002\u4e3a\u4e86\u7406\u89e3\u8fd9\u4e00\u70b9\uff0c\u6211\u4eec\u9700\u8981\u5148\u4e86\u89e3PAGE\u7684\u57fa\u672c\u539f\u7406\u3002PAGE\u662f\u5206\u79bb\u86cb\u767d\u8d28\u6216\u591a\u80bd\u7684\u91cd\u8981\u6280\u672f\u4e4b\u4e00\uff0c\u5176\u6838\u5fc3\u5728\u4e8e\u5229\u7528\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u4f5c\u4e3a\u652f\u6301\u4ecb\u8d28\u6765\u5b9e\u73b0\u6837\u54c1\u7684\u5206\u79bb\u3002\u800c\u5f62\u6210\u8fd9\u79cd\u51dd\u80f6\u7684\u5173\u952e\u6b65\u9aa4\u5c31\u662f\u4e19\u70ef\u9170\u80fa\u5355\u4f53\u7684\u805a\u5408\u8fc7\u7a0b\u3002<\/p>\n
\u5728\u8fd9\u4e2a\u8fc7\u7a0b\u4e2d\uff0cTEMED\u7684\u4f5c\u7528\u4e3b\u8981\u4f53\u73b0\u5728\u4ee5\u4e0b\u51e0\u4e2a\u65b9\u9762\uff1a<\/p>\n
\n
\n
\u52a0\u901f\u81ea\u7531\u57fa\u751f\u6210<\/strong>\uff1a\u5728PAGE\u4f53\u7cfb\u4e2d\uff0c\u901a\u5e38\u4f1a\u52a0\u5165\u8fc7\u786b\u9178\u94f5\uff08APS\uff09\u4f5c\u4e3a\u5f15\u53d1\u5242\u3002\u7136\u800c\uff0c\u4ec5\u4ec5\u4f9d\u9760APS\u5e76\u4e0d\u80fd\u5feb\u901f\u6709\u6548\u5730\u542f\u52a8\u805a\u5408\u53cd\u5e94\u3002\u6b64\u65f6\uff0cTEMED\u4fbf\u767b\u573a\u4e86\u2014\u2014\u5b83\u80fd\u591f\u4e0eAPS\u76f8\u4e92\u4f5c\u7528\uff0c\u4fc3\u8fdb\u81ea\u7531\u57fa\u7684\u751f\u6210\uff0c\u4ece\u800c\u52a0\u5feb\u4e19\u70ef\u9170\u80fa\u5355\u4f53\u4e4b\u95f4\u7684\u4ea4\u8054\u901f\u5ea6\u3002<\/p>\n<\/li>\n
\n
\u8c03\u8282\u51dd\u80f6\u5b54\u5f84\u5927\u5c0f<\/strong>\uff1a\u901a\u8fc7\u8c03\u6574TEMED\u7684\u7528\u91cf\uff0c\u53ef\u4ee5\u63a7\u5236\u805a\u5408\u53cd\u5e94\u7684\u901f\u5ea6\u4ee5\u53ca\u7ec8\u5f62\u6210\u7684\u51dd\u80f6\u5b54\u5f84\u5927\u5c0f\u3002\u8fd9\u5bf9\u4e8e\u4f18\u5316\u7279\u5b9a\u86cb\u767d\u8d28\u7684\u5206\u79bb\u6548\u679c\u81f3\u5173\u91cd\u8981\u3002\u60f3\u8c61\u4e00\u4e0b\uff0c\u5982\u679c\u628a\u51dd\u80f6\u6bd4\u4f5c\u4e00\u5f20\u7b5b\u5b50\uff0c\u90a3\u4e48\u4e0d\u540c\u7684\u5b54\u5f84\u5c31\u76f8\u5f53\u4e8e\u4e0d\u540c\u5c3a\u5bf8\u7684\u7f51\u773c\uff0c\u53ea\u6709\u5408\u9002\u7684\u7f51\u773c\u624d\u80fd\u51c6\u786e\u7b5b\u9009\u51fa\u76ee\u6807\u5206\u5b50\u3002<\/p>\n<\/li>\n<\/ol>\n
\u5b9e\u9a8c\u6761\u4ef6\u5bf9\u6bd4\u8868<\/h3>\n\n\n\n\n\n\n\n
\u5b9e\u9a8c\u53d8\u91cf<\/th>\n \u4e0d\u52a0TEMED\u65f6\u7684\u7ed3\u679c<\/th>\n \u52a0\u5165\u9002\u91cfTEMED\u540e\u7684\u7ed3\u679c<\/th>\n<\/tr>\n<\/thead>\n
\u805a\u5408\u65f6\u95f4<\/td>\n \u9700\u8981\u6570\u5c0f\u65f6\u751a\u81f3\u66f4\u957f\u65f6\u95f4<\/td>\n \u51e0\u5206\u949f\u5185\u5373\u53ef\u5b8c\u6210\u805a\u5408<\/td>\n<\/tr>\n
\u51dd\u80f6\u5747\u5300\u6027<\/td>\n \u53ef\u80fd\u51fa\u73b0\u4e0d\u5747\u5300\u73b0\u8c61<\/td>\n \u51dd\u80f6\u66f4\u52a0\u5747\u5300\u81f4\u5bc6<\/td>\n<\/tr>\n
\u5206\u79bb\u6548\u7387<\/td>\n \u8f83\u4f4e<\/td>\n \u663e\u8457\u63d0\u9ad8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\u6b63\u5982\u8868\u683c\u6240\u793a\uff0c\u52a0\u5165TEMED\u540e\uff0c\u5b9e\u9a8c\u6548\u7387\u5f97\u5230\u4e86\u663e\u8457\u63d0\u5347\uff0c\u540c\u65f6\u7ed3\u679c\u4e5f\u66f4\u52a0\u53ef\u9760\u3002<\/p>\n
\u5176\u4ed6\u5e94\u7528\u9886\u57df<\/h2>\n
\u9664\u4e86\u5728PAGE\u4e2d\u7684\u5e7f\u6cdb\u5e94\u7528\u5916\uff0cTEMED\u8fd8\u5728\u5176\u4ed6\u591a\u4e2a\u9886\u57df\u5c55\u73b0\u4e86\u5176\u72ec\u7279\u7684\u4ef7\u503c\uff1a<\/p>\n
\n
DNA\/RNA\u7814\u7a76<\/strong>\uff1a\u5728\u4e00\u4e9b\u6838\u9178\u76f8\u5173\u5b9e\u9a8c\u4e2d\uff0c\u5982\u975e\u53d8\u6027\u51dd\u80f6\u7535\u6cf3\uff0cTEMED\u540c\u6837\u88ab\u7528\u6765\u4fc3\u8fdb\u51dd\u80f6\u7684\u5f62\u6210\u3002<\/li>\n
\u6750\u6599\u79d1\u5b66<\/strong>\uff1a\u7531\u4e8e\u5176\u5f3a\u5927\u7684\u50ac\u5316\u80fd\u529b\uff0cTEMED\u4e5f\u88ab\u7528\u4e8e\u67d0\u4e9b\u529f\u80fd\u6027\u9ad8\u5206\u5b50\u6750\u6599\u7684\u5408\u6210\u8fc7\u7a0b\u4e2d\u3002<\/li>\n
\u836f\u7269\u5f00\u53d1<\/strong>\uff1a\u5728\u67d0\u4e9b\u65b0\u578b\u836f\u7269\u8f7d\u4f53\u7684\u8bbe\u8ba1\u4e2d\uff0cTEMED\u53ef\u80fd\u53c2\u4e0e\u5176\u4e2d\uff0c\u5e2e\u52a9\u6784\u5efa\u5177\u6709\u7279\u5b9a\u6027\u8d28\u7684\u7eb3\u7c73\u9897\u7c92\u6216\u5176\u4ed6\u9012\u9001\u7cfb\u7edf\u3002<\/li>\n<\/ul>\n
\u5b89\u5168\u4f7f\u7528\u987b\u77e5<\/h2>\n
\u5c3d\u7ba1TEMED\u5728\u5b9e\u9a8c\u4e2d\u6709\u8bf8\u591a\u4f18\u70b9\uff0c\u4f46\u5176\u6bd2\u6027\u4e5f\u4e0d\u5bb9\u5ffd\u89c6\u3002\u957f\u671f\u63a5\u89e6\u6216\u5438\u5165\u9ad8\u6d53\u5ea6\u7684TEMED\u84b8\u6c7d\u53ef\u80fd\u4f1a\u5bf9\u4eba\u4f53\u5065\u5eb7\u9020\u6210\u635f\u5bb3\uff0c\u56e0\u6b64\u5728\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u52a1\u5fc5\u91c7\u53d6\u9002\u5f53\u7684\u5b89\u5168\u9632\u62a4\u63aa\u65bd\uff0c\u4f8b\u5982\u4f69\u6234\u624b\u5957\u3001\u62a4\u76ee\u955c\uff0c\u5e76\u786e\u4fdd\u5b9e\u9a8c\u533a\u57df\u901a\u98ce\u826f\u597d\u3002<\/p>\n
\u5e38\u89c1\u95ee\u9898\u89e3\u7b54<\/h3>\n
Q: TEMED\u662f\u5426\u53ef\u4ee5\u91cd\u590d\u4f7f\u7528\uff1f<\/strong><\/p>\n
A: \u4e0d\u5efa\u8bae\u91cd\u590d\u4f7f\u7528\u3002\u56e0\u4e3a\u6bcf\u6b21\u4f7f\u7528\u540e\uff0cTEMED\u7684\u6709\u6548\u6210\u5206\u53ef\u80fd\u4f1a\u6709\u6240\u635f\u8017\uff0c\u5f71\u54cd\u540e\u7eed\u5b9e\u9a8c\u7684\u6548\u679c\u3002<\/p>\n
Q: \u5982\u679c\u4e0d\u5c0f\u5fc3\u8bef\u670d\u4e86\u5c11\u91cfTEMED\u600e\u4e48\u529e\uff1f<\/strong><\/p>\n
A: \u7acb\u5373\u6f31\u53e3\u5e76\u996e\u7528\u5927\u91cf\u6e05\u6c34\u7a00\u91ca\uff0c\u968f\u540e\u5c3d\u5feb\u5c31\u533b\u54a8\u8be2\u4e13\u4e1a\u533b\u751f\u7684\u610f\u89c1\u3002<\/p>\n
\u7ed3\u8bed<\/h2>\n
\u7efc\u4e0a\u6240\u8ff0\uff0c\u56db\u7532\u57fa\u4e59\u4e8c\u80fa\uff08TEMED\uff09\u867d\u7136\u53ea\u662f\u751f\u7269\u5316\u5b66\u5b9e\u9a8c\u4e2d\u7684\u4e00\u4e2a\u5c0f\u89d2\u8272\uff0c\u4f46\u5b83\u7684\u91cd\u8981\u6027\u5374\u4e0d\u5bb9\u5c0f\u89d1\u3002\u65e0\u8bba\u662f\u4f5c\u4e3aPAGE\u5b9e\u9a8c\u4e2d\u7684\u50ac\u5316\u5242\uff0c\u8fd8\u662f\u5728\u5176\u4ed6\u79d1\u7814\u9886\u57df\u7684\u8f85\u52a9\u5de5\u5177\uff0cTEMED\u90fd\u4ee5\u5176\u72ec\u7279\u7684\u65b9\u5f0f\u63a8\u52a8\u7740\u79d1\u5b66\u7814\u7a76\u5411\u524d\u53d1\u5c55\u3002\u6b63\u5982\u4e00\u9996\u4e50\u66f2\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u97f3\u7b26\uff0c\u6216\u8005\u4e00\u5e45\u753b\u4f5c\u4e2d\u6070\u5230\u597d\u5904\u7684\u4e00\u7b14\uff0cTEMED\u7684\u5b58\u5728\u8ba9\u6574\u4e2a\u5b9e\u9a8c\u6d41\u7a0b\u53d8\u5f97\u66f4\u52a0\u6d41\u7545\u9ad8\u6548\u3002\u5e0c\u671b\u672c\u6587\u80fd\u591f\u5e2e\u52a9\u4f60\u66f4\u597d\u5730\u7406\u89e3\u548c\u8fd0\u7528\u8fd9\u4e00\u795e\u5947\u7684\u5316\u5b66\u54c1\uff0c\u5728\u672a\u6765\u7684\u79d1\u7814\u9053\u8def\u4e0a\u53d6\u5f97\u66f4\u591a\u4e30\u7855\u6210\u679c\uff01<\/p>\n
\n
\u53c2\u8003\u6587\u732e<\/strong><\/p>\n
\n
Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-685.<\/li>\n
Scopes, R.K. Protein Purification: Principles and Practice. Springer Science & Business Media; 2013.<\/li>\n
Sambrook, J., Russell, D.W. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press; 2001.<\/li>\n
Wilson, K., Walker, J. Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press; 2018.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"
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